FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947904

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947904
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16045071
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG3197401.9927615153588292Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAAC619930.38636787584174603TruSeq Adapter, Index 25 (97% over 38bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACAA471920.2941214781785634TruSeq Adapter, Index 25 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACAAT462880.28848734916785346TruSeq Adapter, Index 25 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACACT445760.2778174057316418TruSeq Adapter, Index 25 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACATA430340.26820697770673624TruSeq Adapter, Index 25 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACAAA429340.2675837333471444TruSeq Adapter, Index 25 (97% over 38bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA422960.2636074343329487No Hit
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACAT418920.26108952712019784TruSeq Adapter, Index 25 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACACA376090.23439597119888095TruSeq Adapter, Index 25 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACAAC372180.231959085752877TruSeq Adapter, Index 25 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACATC370720.23104914898787296TruSeq Adapter, Index 25 (97% over 38bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACAC363700.2266739735835385TruSeq Adapter, Index 25 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACATT338710.21109909703733937TruSeq Adapter, Index 25 (97% over 38bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACA283660.17678949504181066TruSeq Adapter, Index 25 (97% over 38bp)
CGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGG276030.17203414057812522No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA270520.1686000641567744No Hit
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACACC263770.16439316472952972TruSeq Adapter, Index 25 (97% over 38bp)
CTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC241850.1507316483672774No Hit
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACAGC225590.1405976950803147TruSeq Adapter, Index 25 (97% over 38bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACAG192140.11975017125196889TruSeq Adapter, Index 25 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACAAG190950.11900851046405467TruSeq Adapter, Index 25 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACAGT188360.1173943075727119TruSeq Adapter, Index 25 (97% over 38bp)
GGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGAT185280.11547471494516914No Hit
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGG176980.11030178676055717TruSeq Adapter, Index 5 (97% over 37bp)
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC175420.10932952555959398No Hit
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACATG171280.1067492939108839TruSeq Adapter, Index 25 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACAGA164140.10229932918339843TruSeq Adapter, Index 25 (97% over 38bp)
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGAC163870.10213105320630866No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)