FastQCFastQC Report
Sun 5 May 2024
EGAF00007947905

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947905
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16045071
Total Bases2.4 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG3493422.177254310685194Illumina Single End PCR Primer 1 (100% over 30bp)
CTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGAGAACGTGTA1706261.0634169209971087Illumina Single End PCR Primer 1 (97% over 34bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG945970.5895704668430573No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGAGAACGTGTAGATC353430.22027325401053072Illumina Single End PCR Primer 1 (97% over 34bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGT347250.21642160386825338No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA316620.19733162913395647No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT311400.19407829357688727No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT274750.1712363877978477No Hit
CGTATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGAGAACGTG256710.15999305955081158Illumina Single End PCR Primer 1 (97% over 34bp)
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA236810.14759049679493472No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG229370.14295355875957172No Hit
CGTATGCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGAGAAC215030.13401623464302526Illumina Single End PCR Primer 1 (97% over 34bp)
CGTATGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGAGAACGT198180.12351456718390338Illumina Single End PCR Primer 1 (97% over 34bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGG184450.11495742212670795No Hit
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGAGAACGTGT184250.11483277325478958Illumina Single End PCR Primer 1 (97% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)