FastQCFastQC Report
Fri 10 May 2024
EGAF00007947913

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947913
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5645251
Total Bases852.4 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG159690.28287493328463165No Hit
GGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTTGGACTGGTGTAG133150.23586196610212726Illumina Single End Sequencing Primer (100% over 33bp)
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT103450.1832513735881717No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG95200.16863732011207297Illumina Single End PCR Primer 1 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG77080.13653954447729605No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA66740.1182232641205856No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA64180.11368847904194163No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)