FastQCFastQC Report
Thu 9 May 2024
EGAF00007947914

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947914
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27426382
Total Bases4.1 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGAAA2287280.8339707366432803TruSeq Adapter, Index 12 (97% over 38bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1894500.6907582633392914No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGCAA1829040.6668907331634191TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGAAC1756410.6404089318087964TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGAAT1628710.5938479235066441TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGCTA1501050.5473014997020023TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGTAA1428880.5209874200687499TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGCAC1417430.5168126076563799TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGCAT1397940.5097063112444069TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGATA1353310.49343365814710816TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGACA1300240.4740836760751017TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGCTC1228970.44809774763583476TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGTAC1207080.440116381373234TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGTAT1079690.39366840292678784TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGCCA1079350.3935444346979488TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGTCA1015980.37043894451699827TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGTCT991670.36157521615501453TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGACC985520.3593328496627809TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGCTT948720.34591511195315516TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGAAG939300.34248046278944116TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGCCC913570.33309898476583605TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGGAA911400.33230777577589343TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGATT909630.3316624117610555TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGTTA904060.32963152048272354TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGACT892720.32549681543850734TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGATC790320.28816050181172276TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGAGC769870.28070417745949866TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGTGC768360.2801536126784787TruSeq Adapter, Index 12 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGAA742180.2706080590578809TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGAGA736960.26870478213276544TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGGAC736580.26856622940641606TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGGCA728510.2656238070336802TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGATG721750.263159026954412TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGGCT720080.26255012418335016TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGCAG684510.2495808597721712TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGCCT669440.24408615033510433TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGGTA657660.23979101581827308TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGTGA653830.23839455018164626TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGTGT641620.23394263231657753TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGACG638210.23269930390381055TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGCGC618820.22562946873561376TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGGAT617540.22516276481527897TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGCGA609530.2222422191888088TruSeq Adapter, Index 12 (97% over 38bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG608750.22195782148735477Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGCTG605980.22094784503475523TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGTCC605720.2208530458009372TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGTTT600650.21900446074148605TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGGCC576770.2102975157277398TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGAGT568940.20744260034006673TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGTAG564020.2056487071462798TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGCGT555440.20252033243028555TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGTTC538330.19628181362018512TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGGTC491120.17906846043346147TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGAGG487080.17759542618490473TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGGTG469490.17118189340467876TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGTTG463990.16917652499699012TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGGTT448070.16337189498782595TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGGGC407250.14848841527839873TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGTGG377860.13777245573258623TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGCGG366710.13370702705154475TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGGGA340040.12398281333644372TruSeq Adapter, Index 12 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGTA324320.11825110581483186TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGTCG310840.11333613015380593TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGGCG295220.10764088387597022TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGGGT293560.10703562722928603TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGGAG288710.10526725690614243TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGCCG277620.10122370497136661TruSeq Adapter, Index 12 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGCA276330.10075335492665419TruSeq Adapter, Index 12 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)