FastQCFastQC Report
Fri 10 May 2024
EGAF00007947924

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947924
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13490633
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG986550.7312851813550928Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGTGT359310.2663403563050007TruSeq Adapter, Index 9 (97% over 36bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGTGTGC182300.13513079779132678TruSeq Adapter, Index 9 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGTGTGCA146080.10828253944792658TruSeq Adapter, Index 9 (97% over 36bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGTGTGA135110.10015097141846493TruSeq Adapter, Index 9 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)