FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947925

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947925
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13490633
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG1080080.8006147672981692Illumina Single End PCR Primer 1 (100% over 30bp)
CGTATGCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAGCCATG180710.1339522022428451Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG144550.10714841920316119No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)