FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947930

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947930
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13121092
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG1778401.3553749947031848Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTGTGT331340.2525247136442607TruSeq Adapter, Index 12 (97% over 38bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTGTGTAA221940.16914750693006345TruSeq Adapter, Index 12 (97% over 38bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA196090.14944640278415852No Hit
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTGTGTAAA188090.14334934927672177TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTGTGTAAAA143240.10916774305065463TruSeq Adapter, Index 12 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)