FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947936

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947936
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24812202
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG3136181.2639668176165904Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2482081.0003465230534556No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTCTC819140.33013595488219866TruSeq Adapter, Index 7 (97% over 35bp)
AAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT694600.2799429087349845Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTTCT600830.24215101908327202TruSeq Adapter, Index 7 (97% over 35bp)
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG596400.24036560721212896No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTACT559260.22539716547527702TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTGCT541880.2183925473442462TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTCTT517860.2087118265440528TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTTAC502210.20240444600604168TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTCTA501380.20206993317239638TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTGCC496990.20030064240166998TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTCCC495840.19983716076469152TruSeq Adapter, Index 7 (97% over 35bp)
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA482170.19432777469730417No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTCAC471330.18995895648439426TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTTTC460840.18573119790012996TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTAAC452930.18254325029273905TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTACC420520.16948112868015502TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTTCC417590.168300258074636TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTGCA412650.16630930217318077TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTCCT402700.16229917844454111TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTCAT395090.159232139090275TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTCAA384580.15499631995580238TruSeq Adapter, Index 7 (97% over 35bp)
CACACACACACACACACACACACACACACACACACACACACACACACACA382030.1539685998042415No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTTTT381080.15358572366934625TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTTAA372280.15003908157768506TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTTAT369300.14883805959664523TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTATC358750.14458611936175597TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTTTA358550.14450551385967275TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTACA345860.13939109475249314TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTTCA333350.13434922059718843TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTCCA332760.13411143436604295TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTTGC326300.13150787664875532TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTAAT319140.12862219967417643TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTAAA314950.12693351440553322TruSeq Adapter, Index 7 (97% over 35bp)
AGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC309500.12473701447376577Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTGTC296130.11934853665950325TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTATT295060.11891729722335809TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTATA259470.10457354812765107TruSeq Adapter, Index 7 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)