FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947937

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947937
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24812202
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG3355821.3524877800043704Illumina Single End PCR Primer 1 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1595570.643058604794528No Hit
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGCGTGT862190.34748628920560937Illumina Single End PCR Primer 1 (97% over 35bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG810670.32672231186897477No Hit
CGTATGCCGTCTTCTGCTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT736110.2966725806923545Illumina Single End PCR Primer 1 (100% over 31bp)
CACACACACACACACACACACACACACACACACACACACACACACACACA474530.19124864451772558No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA405940.1636049875782891No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGT312040.12576070435022252No Hit
CGTATGCCGTCTTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAG294190.11856666328929613Illumina Single End PCR Primer 1 (100% over 35bp)
CCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC256450.10335640504619462No Hit
CGTATGCCGTCTTCTGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG249040.10036997119401171Illumina Single End PCR Primer 1 (100% over 32bp)
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG248340.10008785193672048No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)