FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947938

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947938
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13192310
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGACAATCAGCCTATGAGATCGGAAGAGCACACGTCTGAACTCCA548570.4158255832375073Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA408040.30930140362074576No Hit
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG404070.3062920747010948Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGG243110.18428160041721275No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC212480.16106352867693377No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT189780.14385653460235545No Hit
CTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC166320.12607344733409084No Hit
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGAC149960.11367228332263267No Hit
CGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAA139490.10573584156224347No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)