FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947940

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947940
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14074452
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGCAA948870.6741790017828048TruSeq Adapter, Index 8 (97% over 38bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA774230.5500960179479812No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGCTA738370.5246172284363185TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGCAC724300.5146203916145368TruSeq Adapter, Index 8 (97% over 38bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG600840.42690116815915813Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGCTC598490.4252314761526772TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGCCA595720.42326337110674006TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGCAT586010.41636434583740806TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGACT572340.40665171191034655TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGACA558200.39660513958198873TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGAAA495920.3523547488740592TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGCCC476760.3387414302169633TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGCCT470110.3340165570922406TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGAAC469330.3334623614475363TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGCTT460370.3270962166058046TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGACC451550.32082954277722503TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGATC382110.27149192025380453TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGATA377670.2683372681224107TruSeq Adapter, Index 8 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGAA377530.26823779710925866TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGTAC353290.25101510168921676TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGAAT347590.24696521043945438TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGTCT339550.24125273225557914TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGTAA331500.23553314899933583TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGTCA268150.19052251554802985TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGATT253780.18031252655520796TruSeq Adapter, Index 8 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGCA252130.1791401896144873TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGGCT239830.17040095060184224TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGGCA235340.1672107731086084TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGTAT226000.16057463551689258TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGCGA221210.15717130585261863TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGAGC218170.1550113638527454TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGGCC206570.14676947990586062TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGGAC201850.1434158857481627TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGTTA198200.1408225343338412TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGCTG196310.13947967565628844TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGTCC191600.13613318657095852TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGAGA188310.13379561776188514TruSeq Adapter, Index 8 (97% over 38bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGA170280.12098517228237375TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGCAG170140.12088570126922171TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGGAT164060.11656581726947521TruSeq Adapter, Index 8 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGTA162360.1153579549669145TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGGAA159520.11334011441440137TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGTTC156850.11144306009214426TruSeq Adapter, Index 8 (97% over 38bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGC152030.1080184152107663TruSeq Adapter, Index 8 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGAT149580.1062776724806053TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGTTT145230.1031869660005235TruSeq Adapter, Index 8 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)