FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947942

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947942
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25537123
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG2060510.8068684949357842Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCAAA1442930.5650323256852388TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCACT1240860.48590438319931345TruSeq Adapter, Index 1 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCAA1171970.45892796929395685TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCACA1135960.4448269290162404TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCAAT1123800.4400652336600329TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCAAC1096300.4292965969580833TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCTAA1040680.4075165397449039TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCATA976450.3823649202770414TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCCAA949740.3719056371385297TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCGCA892100.3493345746112434TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCTCT840800.32924617232724296TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCGAA839440.32871361429398294TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCTTA812710.31824649941968797TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCGCT810770.31748682104871406TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCTAC791970.3101249894124722TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCACC788520.30877401498986395TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCATC763250.29887861682774525TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCCTC747690.2927855263883876TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCCTA710700.2783007310572926TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCCAC690980.2705786395750218TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCTCA687400.26917675886982256TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCATT687250.2691180208514483TruSeq Adapter, Index 1 (97% over 36bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA668070.261607386235325No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCCAT657970.2576523596647908TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCCCA654490.25628963763850765TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCTAT650110.254574487501979TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCAAG648260.25385005194202964TruSeq Adapter, Index 1 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCTA641230.25109719681422216TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCTGC597290.23389087329845262TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCTTT590620.2312789894147434TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCGAC585120.2291252620743535TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCCCT576970.2259338297426848TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCTGA575250.2252603004653265TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCAGA565290.22136009604527496TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCGTA564720.22113689157545274TruSeq Adapter, Index 1 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCAC561100.219719347398687TruSeq Adapter, Index 1 (97% over 36bp)
ACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGT561000.2196801887197708TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCGAT556670.2179846179227002TruSeq Adapter, Index 1 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCAT543050.2126512058543165TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCATG524840.20552041042368008TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCGCC512480.2006803977096402TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCTTC479600.18780502408200014TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCCCC478140.18723330736982394TruSeq Adapter, Index 1 (97% over 36bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCA468550.18347799006176224TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCGTC464580.1819233905087899TruSeq Adapter, Index 1 (97% over 36bp)
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA458470.17953079522701126No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCTCC449620.17606525214292934TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCTGT441950.1730617814700583TruSeq Adapter, Index 1 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCCA428340.16773228526956618TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCAGC427490.16739943649877867TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCCTT426580.16704309252064142TruSeq Adapter, Index 1 (97% over 36bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTT411560.16116145894743114TruSeq Adapter, Index 1 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCGA410830.16087560059134304TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCACG373730.1463477307134402TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCAGT359880.14092425368354922TruSeq Adapter, Index 1 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCGC357840.14012541663365916TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCTAG345760.1353950482205846TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCGTT345100.1351366009397378TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCTTG342040.1339383453649027TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCGGA325450.12744192053270842TruSeq Adapter, Index 1 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCTT319500.12511197913719568TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCGCG318150.12458333697182725TruSeq Adapter, Index 1 (97% over 36bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCT314100.12299741047572196TruSeq Adapter, Index 1 (97% over 36bp)
CGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGG306960.12020148080110668No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCGTG304730.11932824226127588TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCCGC300360.1176170079926388TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCGAG299360.117225421203477TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCCAG297550.11651664911509413TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCTCG294740.11541629023754947TruSeq Adapter, Index 1 (97% over 36bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCA291500.11414754904066522TruSeq Adapter, Index 1 (96% over 32bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCCGA276530.10828549480691306TruSeq Adapter, Index 1 (97% over 36bp)
AAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAC272530.10671914765026584TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCGGC272140.10656642880249274TruSeq Adapter, Index 1 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCCT269530.10554438728278046TruSeq Adapter, Index 1 (97% over 36bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTC268020.10495309123114613TruSeq Adapter, Index 1 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)