FastQCFastQC Report
Wed 8 May 2024
EGAF00007947962

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947962
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8380423
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA406870.485500552895719No Hit
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG315970.37703347432462536Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG171680.2048583943793768No Hit
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTACTCTCAA88610.10573451960599126TruSeq Adapter, Index 18 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)