FastQCFastQC Report
Thu 9 May 2024
EGAF00007947964

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947964
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6464503
Total Bases976.1 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG3075404.757364951335006Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCT1537232.377955428282731TruSeq Adapter, Index 19 (97% over 37bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTT1442942.2320973476228567TruSeq Adapter, Index 19 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTA1081871.6735547960918264TruSeq Adapter, Index 19 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTT985621.5246647731465204TruSeq Adapter, Index 19 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTC781141.2083527534908716TruSeq Adapter, Index 19 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTG629930.9744445938071342TruSeq Adapter, Index 19 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTAC599960.9280837212079568TruSeq Adapter, Index 19 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTTA518230.801654821724114TruSeq Adapter, Index 19 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTTT500700.774537501181452TruSeq Adapter, Index 19 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTCT492110.7612495500427487TruSeq Adapter, Index 19 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTAT465800.7205503655888164TruSeq Adapter, Index 19 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTAA459230.7103871712953029TruSeq Adapter, Index 19 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTTC399040.6172786987646227TruSeq Adapter, Index 19 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTCA398970.6171704151115717TruSeq Adapter, Index 19 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTCC360220.5572276786011238TruSeq Adapter, Index 19 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTGC358260.5541957363156921TruSeq Adapter, Index 19 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTGA309770.47918610293784375TruSeq Adapter, Index 19 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTTG288840.4468092906755554TruSeq Adapter, Index 19 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTAG274220.4241934762811619TruSeq Adapter, Index 19 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTGT260850.4035112985483958TruSeq Adapter, Index 19 (97% over 37bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGA260780.4034030148953446TruSeq Adapter, Index 19 (100% over 37bp)
GACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAG184040.2846931929647183TruSeq Adapter, Index 19 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTACT173350.2681567322344811TruSeq Adapter, Index 19 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTGG149140.23070605737208258TruSeq Adapter, Index 19 (97% over 37bp)
ACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGC147450.2280917806055624TruSeq Adapter, Index 19 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTACC142370.22023348121270886TruSeq Adapter, Index 19 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTCG139030.2150668040528406TruSeq Adapter, Index 19 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTCTT138440.21415412754855245TruSeq Adapter, Index 19 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTTCT138320.2139684984290362TruSeq Adapter, Index 19 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTAAC132710.20529033709165267TruSeq Adapter, Index 19 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTTAC130330.2016086928879142TruSeq Adapter, Index 19 (97% over 37bp)
GGATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTA125410.19399789898774894TruSeq Adapter, Index 19 (97% over 37bp)
GGATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTT123420.1909195494224382TruSeq Adapter, Index 19 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTTTC116900.18083370059538995TruSeq Adapter, Index 19 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTTTT112620.17421292866597787TruSeq Adapter, Index 19 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTCTA110730.1712892700335973TruSeq Adapter, Index 19 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTTAT108920.1684893641475609TruSeq Adapter, Index 19 (97% over 37bp)
AGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG107930.16695792391155206Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTCCC107040.16558117460847338TruSeq Adapter, Index 19 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTCAT105360.16298236693524623TruSeq Adapter, Index 19 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTATT104920.1623017268303534TruSeq Adapter, Index 19 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTGCT104740.16202328315107906TruSeq Adapter, Index 19 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTCCT103910.1607393484077585TruSeq Adapter, Index 19 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTACA100950.15616049679302493TruSeq Adapter, Index 19 (97% over 37bp)
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC100590.15560360943447624Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTCAC99540.15397935463870926TruSeq Adapter, Index 19 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTATC98240.15196837251061682TruSeq Adapter, Index 19 (97% over 37bp)
GGATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTC97150.15028224134167778TruSeq Adapter, Index 19 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTTTA92530.14313552024030307TruSeq Adapter, Index 19 (97% over 37bp)
CAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC92000.14231565829577308Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTAAT90990.14075327987317818TruSeq Adapter, Index 19 (97% over 37bp)
ATACGAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCT88540.13696335201638857TruSeq Adapter, Index 19 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTTAA87610.13552472634013785TruSeq Adapter, Index 19 (97% over 37bp)
GAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGT87480.13532362812732857TruSeq Adapter, Index 18 (100% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTCTC87140.13479767895536596TruSeq Adapter, Index 19 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTGCC87060.13467392620902177TruSeq Adapter, Index 19 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTGAC84950.13140994752419483TruSeq Adapter, Index 19 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTATA83500.1291669289967071TruSeq Adapter, Index 19 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTTCC78230.12101471683128619TruSeq Adapter, Index 19 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTTCA77890.12048876765932354TruSeq Adapter, Index 19 (97% over 37bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCCG77630.12008657123370504Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CATACGAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGC75780.11722478897449656TruSeq Adapter, Index 19 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTCCA74670.1155077196189715TruSeq Adapter, Index 19 (97% over 37bp)
GGATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTG74200.1147806722341996TruSeq Adapter, Index 19 (97% over 37bp)
GGCATAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTA73980.11444035218175319TruSeq Adapter, Index 19 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTAGC72450.1120735809079213TruSeq Adapter, Index 19 (97% over 37bp)
CAAGCAGAAGACGGATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT71180.11010900605970791Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CAAGAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT70370.1088560095029734Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGCATAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTT69500.10751019838648075TruSeq Adapter, Index 19 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTCAA69130.10693784193463907TruSeq Adapter, Index 19 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTTGC68830.10647376913584848TruSeq Adapter, Index 19 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTAAA68600.10611797999010907TruSeq Adapter, Index 19 (97% over 37bp)
CAAGCAGAAGAGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT67480.10438544154129095Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTG67300.10410699786201662TruSeq Adapter, Index 19 (100% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)