FastQCFastQC Report
Fri 10 May 2024
EGAF00007947965

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947965
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6464503
Total Bases976.1 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC66

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG3168294.901057358933858Illumina Single End PCR Primer 1 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG876491.355850558039806No Hit
CAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGGATCAAGTGTAGAT608310.9410004141076276Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGGATCAA562590.8702757195719455Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGGATCAAG438750.6787064682312004Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGGATCAAGT404200.6252607509038204Illumina Single End PCR Primer 1 (97% over 35bp)
CGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGGATCAAGTGTAG367290.568164327559288Illumina Single End PCR Primer 1 (97% over 35bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGGATCAAGTGTAGATC361720.5595480425950765Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGGATCAAGTG341430.5281612523035414Illumina Single End PCR Primer 1 (97% over 35bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGT321320.4970529056912806No Hit
CGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGGATCAAGTGTAGA310790.4807639504537317Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGGATCAAGTGT307510.47569008785362155Illumina Single End PCR Primer 1 (97% over 35bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT206280.3190964564483921No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGG190390.2945160672057852No Hit
CGTATGCCGTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGGAT163650.25315171174025286Illumina Single End PCR Primer 1 (97% over 35bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTT127820.19772595047136649No Hit
CGTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGGATCAAGTGTA118150.18276733725701727Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCGTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGGATC112550.17410464501292675Illumina Single End PCR Primer 1 (97% over 35bp)
CTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGGATCAAGTGTAGA110780.1713666155000624Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCGTCTTCTGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG90810.14047483619390386Illumina Single End PCR Primer 1 (100% over 32bp)
ATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAA77580.12000922576723995Illumina Single End PCR Primer 1 (100% over 26bp)
CGTATCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGGATCAAGT75000.11601819969764111Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCGTCTTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCG74150.11470332676773451Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCGTCTTCTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTC67930.1050815507394768Illumina Single End Sequencing Primer (100% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGTGT65040.10061098277779437No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)