FastQCFastQC Report
Fri 10 May 2024
EGAF00007947967

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947967
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12775861
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG3191022.497694675920472Illumina Single End PCR Primer 1 (100% over 30bp)
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGTAGGGTGT1118790.8757061461454536Illumina Single End Sequencing Primer (100% over 33bp)
CGTATGCCGTCTTCTGCTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT874010.6841104486030335Illumina Single End PCR Primer 1 (100% over 31bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGTAGGGTGTAGATC494500.38705806207503357Illumina Single End Sequencing Primer (100% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG399710.31286345397777887No Hit
CGTATGCCGTCTTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTC272650.2134102742664467Illumina Single End Sequencing Primer (100% over 33bp)
CGTATGCCGTCTTCTGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG196710.15397005336861444Illumina Single End PCR Primer 1 (100% over 32bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGT195420.15296033668494044No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA131740.10311633791256808No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)