FastQCFastQC Report
Wed 1 May 2024
EGAF00007947970

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947970
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20416032
Total Bases3 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA608580.2980892663177644No Hit
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG396170.19404848111523335Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTAAGCTC206940.10136151824213441TruSeq Adapter, Index 25 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)