FastQCFastQC Report
Fri 10 May 2024
EGAF00007947978

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947978
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12376489
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG789430.6378464845724826Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AATGATACGGCGACCACCGAGATCTACACTGGAGTTGACACTCTTTCCCT365430.29526144288578127Illumina Single End PCR Primer 1 (96% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA294450.23791076774681416No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCCATGTGC172120.1390701353186675TruSeq Adapter, Index 9 (97% over 37bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCCAT171770.13878734106255822TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCCATGCTC156880.12675646542407948TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCCATGACT140750.11372369013538493TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCCATGTAC138200.1116633319837314TruSeq Adapter, Index 9 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)