FastQCFastQC Report
Fri 10 May 2024
EGAF00007947979

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947979
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12376489
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1898571.5340134023469822No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG910880.7359760914424115Illumina Single End PCR Primer 1 (100% over 30bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAACTCCAGTGTAGATCT488660.39482926054392325Illumina Single End Sequencing Primer (100% over 32bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT433530.3502851252887632No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGT348750.28178427662320066No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGG312940.2528503843052743No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA177960.1437887594777485No Hit
GAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAACTCCAGTGTAG154360.12472034678009249Illumina Single End Sequencing Primer (100% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAACTCCAGTGTAGATC131190.10599936702565646Illumina Single End Sequencing Primer (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)