FastQCFastQC Report
Wed 8 May 2024
EGAF00007947985

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947985
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7685602
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG99540.12951490332182178Illumina Single End PCR Primer 1 (100% over 30bp)
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT94140.1224887783676542No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA83580.10874880067950435No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)