FastQCFastQC Report
Wed 8 May 2024
EGAF00007947986

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947986
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10981377
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG711680.6480790159558314Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGTCTTA163170.1485879229899857TruSeq Adapter, Index 5 (97% over 38bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA161960.1474860575317649No Hit
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGTCT146060.13300699903117796TruSeq Adapter, Index 5 (97% over 38bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGTCTT122390.11145232515011551TruSeq Adapter, Index 5 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGTCTTAA115640.1053055550319418TruSeq Adapter, Index 5 (97% over 38bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGTCTTT113460.10332037594192423TruSeq Adapter, Index 5 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)