FastQCFastQC Report
Wed 8 May 2024
EGAF00007947988

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947988
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7419021
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG516360.6959947950005803Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGACG262010.35315980369916733TruSeq Adapter, Index 4 (97% over 39bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGACA257510.34709431338717067TruSeq Adapter, Index 4 (97% over 39bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA256140.3452477085588516No Hit
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGAC244680.32980092656430005TruSeq Adapter, Index 4 (97% over 39bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGACC194990.2628244346524966TruSeq Adapter, Index 4 (97% over 39bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGACT187980.2533757486331418TruSeq Adapter, Index 4 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGACGC138810.1871001578240579TruSeq Adapter, Index 4 (97% over 39bp)
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA137010.18467396169925926No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGACCC135420.18253082178902041TruSeq Adapter, Index 4 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGACAC123890.1669896877229489TruSeq Adapter, Index 4 (97% over 39bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGAGC123730.16677402584518902TruSeq Adapter, Index 4 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGACGT113800.1533895105567163TruSeq Adapter, Index 4 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGACGA109900.14813275228631917TruSeq Adapter, Index 4 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGACTC108730.14655572480520004TruSeq Adapter, Index 4 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGACCT106560.14363081058808164TruSeq Adapter, Index 4 (97% over 39bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGAGT100380.13530087055960618TruSeq Adapter, Index 4 (97% over 39bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGAG95900.12926233798232947TruSeq Adapter, Index 4 (97% over 39bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGAC94960.12799532445049017TruSeq Adapter, Index 4 (96% over 33bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGACGG94810.1277931414400903TruSeq Adapter, Index 4 (97% over 39bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGATA90710.1222668058224933TruSeq Adapter, Index 4 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGACTT89900.12117501756633388TruSeq Adapter, Index 4 (97% over 39bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGATC85890.11576999175497685TruSeq Adapter, Index 4 (97% over 39bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGAGG84320.11365380957945798TruSeq Adapter, Index 4 (97% over 39bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGAGA82720.11149719080185917TruSeq Adapter, Index 4 (97% over 39bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAG81400.10971798031034015TruSeq Adapter, Index 4 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGACCA80560.10858575545210077TruSeq Adapter, Index 4 (97% over 39bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGA79780.10753440379802134TruSeq Adapter, Index 4 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGACAT79510.10717047437930152TruSeq Adapter, Index 4 (97% over 39bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGATT78920.10637522120506196TruSeq Adapter, Index 4 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGACTA78400.10567432010234233TruSeq Adapter, Index 4 (97% over 39bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGAT76340.10289767342618386TruSeq Adapter, Index 4 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGACAA75700.10203502591514434TruSeq Adapter, Index 4 (97% over 39bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)