FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947995

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947995
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14971603
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTGAAGTGTA1366300.9125943294114865Illumina Single End PCR Primer 1 (97% over 35bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG272600.18207803132369993No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG249440.16660874590382874Illumina Single End PCR Primer 1 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA248360.1658873802624876No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)