FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007948002

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007948002
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22860079
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG5585752.443451748351351Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTAC1776520.7771276730933432TruSeq Adapter, Index 25 (97% over 37bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTG1399370.6121457410536508TruSeq Adapter, Index 25 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTAA1207750.5283227586396355TruSeq Adapter, Index 25 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTCT1138990.4982441224284483TruSeq Adapter, Index 25 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTA1104520.4831654343801699TruSeq Adapter, Index 25 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTCC1090680.47711121208286283TruSeq Adapter, Index 25 (97% over 37bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGT1079870.47238244452261086TruSeq Adapter, Index 25 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTCA1073140.469438447697403TruSeq Adapter, Index 25 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTGC1072990.46937283112626166TruSeq Adapter, Index 25 (97% over 37bp)
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA1016350.44459601386329417No Hit
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTTA1003410.43893549099283513TruSeq Adapter, Index 25 (97% over 37bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA976710.42725574132967786No Hit
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTTC976690.42724699245352565TruSeq Adapter, Index 25 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTAT939940.411170932523899TruSeq Adapter, Index 25 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTC877410.38381757123411514TruSeq Adapter, Index 25 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTTT835140.36532682148648743TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTACT784090.34299531510805364TruSeq Adapter, Index 25 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTT751660.3288090124272974TruSeq Adapter, Index 25 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTG688900.30135503906176353TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTCTC652890.2856026875497674TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTACC650070.28436909601231036TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTCCT641620.28067269583801524TruSeq Adapter, Index 25 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTGA635620.2780480329923619TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTCCC604360.26437353956650805TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTGCC566390.24776379819159855TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTTCT516870.22610158083880638TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTGCT487110.21308325312436585TruSeq Adapter, Index 25 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTTG486880.2129826410486158TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTACA481820.21076917538211484TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTAAC450000.19684971342399998TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTCTT448580.19622854321719535TruSeq Adapter, Index 25 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTAG431530.18877012629746381TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTCCA419020.1832977042642766TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTCAC415810.18189350964185208TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTCTA400650.17526186151850132TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTTCC399980.17496877416740336TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTTAC397080.17370018712533758TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTCAT388060.1697544439807054TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTGCA382160.16717352551581297TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTTTC379740.16611491150139945TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTCAA379500.1660099249875733TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTTCA377440.165108790743899TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTATC351980.1539714714021767TruSeq Adapter, Index 25 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTCG339920.1486958990824135TruSeq Adapter, Index 25 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTGT338790.14820158757981544TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTTAA324930.14213861640635625TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTAAT319890.13993389961600744TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTGAC315720.1381097589382784TruSeq Adapter, Index 25 (97% over 37bp)
GGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA312780.13682367414390825No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTTTA299770.1311325302069166TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTTGC299350.1309488038077209TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTTTT285930.12507830790960958TruSeq Adapter, Index 25 (97% over 37bp)
CTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC283590.12405468939980478No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTATT283060.12382284418177207TruSeq Adapter, Index 25 (97% over 37bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTG280310.12261987371084762TruSeq Adapter, Index 25 (97% over 37bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTT278850.12198120575173865TruSeq Adapter, Index 6 (96% over 33bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTAAA273240.11952714599105278TruSeq Adapter, Index 25 (97% over 37bp)
ACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTT265570.11617195198669261TruSeq Adapter, Index 25 (97% over 37bp)
CAAGCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGT258230.11296111443884337TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTTAT255910.11194624480519075TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTATA250670.10965403925332017TruSeq Adapter, Index 25 (97% over 37bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTA249830.1092865864549287TruSeq Adapter, Index 6 (96% over 33bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTC243990.10673191461849278TruSeq Adapter, Index 6 (96% over 33bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTGG233970.10234872766625171TruSeq Adapter, Index 25 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)