FastQCFastQC Report
Wed 1 May 2024
EGAF00007948003

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007948003
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22860079
Total Bases3.4 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG5970282.611662015691197Illumina Single End PCR Primer 1 (100% over 30bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTATGCGGTGTGTAGATC2805801.2273798353890202Illumina Single End Sequencing Primer (100% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1141330.49926774093825316No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA801900.35078618932156796No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA700380.3063768939731136No Hit
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTATGCGGTGTGT336200.14706860811810843Illumina Single End Sequencing Primer (100% over 33bp)
CCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC322550.1410975001442471No Hit
CGTATGCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTATGCGGT295680.12934338503379625Illumina Single End Sequencing Primer (100% over 33bp)
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG287330.12569072924026203No Hit
GGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA274380.12002583193172693No Hit
CGTATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTATGCGGTGTG249730.10924284207416782Illumina Single End Sequencing Primer (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)