FastQCFastQC Report
Wed 8 May 2024
EGAF00007948004

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007948004
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences702796
Total Bases106.1 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG28620.4072305476980518Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12680.18042219932953518No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGTCTTCTCT11910.16946596167308864TruSeq Adapter, Index 16 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGTCTTCAA9790.1393007359176774TruSeq Adapter, Index 16 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGTCTTCTGC9380.13346689508762144TruSeq Adapter, Index 16 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGTCTTCACT8410.11966488141651346TruSeq Adapter, Index 16 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGTCTTCTAC8260.1175305494055174TruSeq Adapter, Index 16 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGTCTTCTCA7870.1119812861769276TruSeq Adapter, Index 16 (97% over 38bp)
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA7500.10671660054980393No Hit
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGTCTTCTA7280.10358624693367635TruSeq Adapter, Index 16 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)