FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007948008

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007948008
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24372878
Sequences flagged as poor quality0
Sequence length151
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG13194485.413591287824113Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTAA1649830.6769122628849986TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTCTA1163680.47744874446095364TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTCAA1126420.46216125974125827TruSeq Adapter, Index 8 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTCA1111370.45598636320257296TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTCAT1096780.45000020104314314TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTCTT1063250.43624310596393256TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTTCT956930.3926208468281834TruSeq Adapter, Index 8 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTA941620.3863392743360058TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTACT939190.3853422644629822TruSeq Adapter, Index 8 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTTA915850.3757660461764097TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTCCA905600.37156055185604264TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTCCT899720.36914803413860275TruSeq Adapter, Index 8 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTAT886770.3638347510704317TruSeq Adapter, Index 8 (97% over 37bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA845390.3468568627800131No Hit
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTAC831950.341342536568722TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTAAC800980.3286357893392811TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTACA781440.32061868114221065TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTCAC779060.3196421858756278TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTTTT770380.3160808501975023TruSeq Adapter, Index 8 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTC767580.31493203223681665TruSeq Adapter, Index 8 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTCT737990.30279148814514234TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTATT729490.29930400505020377TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTACC725070.2974905138408357TruSeq Adapter, Index 8 (97% over 37bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCT717500.2943846024256963TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTAAA708380.2906427382108916TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTTAC690910.2834749347204708TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTCTG680970.2793966309600368TruSeq Adapter, Index 8 (97% over 37bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTT669460.27467416855736115TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTAAT661270.2713138760223557TruSeq Adapter, Index 8 (97% over 37bp)
AGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG653200.2680028185428081Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTTTA652470.26770330528877223TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTTCA644340.2643676302814957TruSeq Adapter, Index 8 (97% over 37bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGC641010.2630013574925374TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTTAT629940.25845942362654095TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTTAA629180.2581476016086406TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTTTC613600.25175525024168255TruSeq Adapter, Index 8 (97% over 37bp)
AAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCT592790.24321707104101534TruSeq Adapter, Index 8 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTATA588880.2416128288173436TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTCTC588560.24148153533612238TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTCCC587610.24109175781374692TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTGCT574930.2358892536203562TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTATC569960.23385010174013918TruSeq Adapter, Index 8 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTT565440.23199558131788953TruSeq Adapter, Index 8 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTTT562840.23092882178296711TruSeq Adapter, Index 8 (97% over 37bp)
AAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT562090.2306211026863549Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTCGA559420.22952562270241536TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTCGT511270.20977005669991045TruSeq Adapter, Index 8 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTGA508350.20857200368376685TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTTCC498920.20470294890902913TruSeq Adapter, Index 8 (97% over 37bp)
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC489550.20085851166202037Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAG474310.19460565961885995TruSeq Adapter, Index 8 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTTC432350.17738980189372794TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTCAG423180.17362742307248247TruSeq Adapter, Index 8 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTCC421870.1730899403837331TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGC416950.17107130310995688TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTGAC415430.17044765907415613TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTGAA413910.16982401503835534TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTCCG397990.16329216434759983TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTGCA394890.16202025874826928TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTCGC388930.15957491766052412TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTTGC387360.15893075901828255TruSeq Adapter, Index 8 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTAG381220.15641156534735046TruSeq Adapter, Index 8 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTCG380790.15623513973195943TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTAGC375750.1541672674027253TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTTGA356960.14645787830226698TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTGAT356730.1463635111126392TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTGCC342000.1403199080551751TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTAGA317760.13037442685266795TruSeq Adapter, Index 8 (97% over 37bp)
CAAGCAGAAGACGGCATACGAGAGATCGGAAGAGCACACGTCTGAACTCC315840.12958666596534066Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTAAG299000.12267734651607413TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTCGG293320.12034688722439754TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTTAG288060.1181887506268238TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTTTG287060.11777845849800749TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTGTT276110.113285759687469TruSeq Adapter, Index 8 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTTG275620.113084716544349TruSeq Adapter, Index 8 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTG272070.11162817948705114TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTGTA267820.10988443793958187TruSeq Adapter, Index 8 (97% over 37bp)
CAAGCAGAAGACGGCATACGAGATAGATCGGAAGAGCACACGTCTGAACT264840.10866176739570928Illumina PCR Primer Index 2 (96% over 31bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTTGT263670.1081817256049942TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTATG255810.10495682947249808TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTACG253650.10407059847425486TruSeq Adapter, Index 8 (97% over 37bp)
CAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC248450.1019370794044101Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTGC245000.10052157155999385TruSeq Adapter, Index 8 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)