FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007948009

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007948009
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24372878
Sequences flagged as poor quality0
Sequence length151
%GC63

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG14087415.779953438408053Illumina Single End PCR Primer 1 (100% over 30bp)
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGTCAGTGGTGT2213370.9081282891581373Illumina Single End PCR Primer 1 (97% over 35bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1254100.5145473587485236No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGTCAGTGGTGTAGATC964060.39554622970664355Illumina Single End PCR Primer 1 (97% over 35bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA755840.31011520264451325No Hit
CGTATGCCGTCTTCTGCTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT588740.24155538791930933Illumina Single End PCR Primer 1 (100% over 31bp)
CGTATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGTCAGTGGTG568620.23330031028752532Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCGTCTTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTG563780.23131449638405444Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCGTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGTCA506020.20761602302362486Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCGTCTTCTGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG505170.207267274714131Illumina Single End PCR Primer 1 (100% over 32bp)
CGTATGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGTCAGTGGT444190.18224766069891296Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCGTCTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGTC423490.1737546136324155Illumina Single End PCR Primer 1 (97% over 35bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGT370480.15200502788386336No Hit
ATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAA340320.13963061727876372Illumina Single End PCR Primer 1 (100% over 26bp)
CTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAG335930.13782943483326016Illumina Single End PCR Primer 1 (100% over 28bp)
CGTATGCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGTCAGTG263460.10809556425794278Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGTCAGTGG243740.10000460347768532Illumina Single End PCR Primer 1 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)