FastQCFastQC Report
Wed 1 May 2024
EGAF00007948011

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007948011
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18328085
Total Bases2.7 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA613090.3345084879298628No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG492780.26886605992933793No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG383270.2091162279092442Illumina Single End PCR Primer 1 (100% over 30bp)
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA292420.159547492277562No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG249600.13618444043663044No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT233510.12740556364726593No Hit
GGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGAACGAAGGTGTA225350.12295338001760685Illumina Single End Sequencing Primer (100% over 33bp)
GGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTA211850.11558763504206795No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)