FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007948024

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007948024
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44116690
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4240920.9612960537157254No Hit
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG2234660.5065339217425423Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA1606350.36411389884417894No Hit
GGGGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA1466770.3324750791593839No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA1408380.31923972537377576No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATTCCTCT830180.18817821554608924TruSeq Adapter, Index 6 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATTCCCTC808650.18329797634410017TruSeq Adapter, Index 6 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATTCCCCT805830.18265876247742066TruSeq Adapter, Index 6 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATTCCACT777180.17616462159785787TruSeq Adapter, Index 6 (97% over 37bp)
GGGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG754310.17098064247340405No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATTCCCAA725010.16433916506428745TruSeq Adapter, Index 6 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATTCCCAC723960.16410115990116214TruSeq Adapter, Index 6 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATTCCAAC692040.15686580294215183TruSeq Adapter, Index 6 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATTCCCCA685120.15529723558136388TruSeq Adapter, Index 6 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATTCCACC678830.15387147131845114TruSeq Adapter, Index 6 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATTCCCCC671920.15230517067350247TruSeq Adapter, Index 6 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATTCCCAT655000.1484698874734256TruSeq Adapter, Index 6 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATTCCACA628180.14239055559245264TruSeq Adapter, Index 6 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATTCCCTT626630.14203921463736285TruSeq Adapter, Index 6 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATTCCAAA595940.13508266372658512TruSeq Adapter, Index 6 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATTCCCTA595260.13492852704951344TruSeq Adapter, Index 6 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATTCCTAC591780.1341397099374409TruSeq Adapter, Index 6 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATTCCATC587680.13321035644333243TruSeq Adapter, Index 6 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATTCCAA574260.1301684237870067TruSeq Adapter, Index 6 (97% over 37bp)
GCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC562590.12752316640255648No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATTCCTAA555170.1258412632498041TruSeq Adapter, Index 6 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATTCCTCA523060.11856283868984731TruSeq Adapter, Index 6 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATTCCATT512550.11618052034275464TruSeq Adapter, Index 6 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATTCCTTC510450.11570451001650396TruSeq Adapter, Index 6 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATTCCATA509440.11547557171673578TruSeq Adapter, Index 6 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATTCCTTT487830.11057719878803238TruSeq Adapter, Index 6 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATTCCTAT485980.11015785635776391TruSeq Adapter, Index 6 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATTCCAAT483930.10969317961070969TruSeq Adapter, Index 6 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATTCCTTA462290.10478800653448841TruSeq Adapter, Index 6 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATTCCTCC458260.10387452005125497TruSeq Adapter, Index 6 (97% over 37bp)
CGGGGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG447360.10140379978643002No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)