FastQCFastQC Report
Wed 15 May 2024
EGAF00007948028

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007948028
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52423457
Total Bases7.9 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1253760.23916011490810307No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT1105380.21085599143146933No Hit
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG721670.13766165783382045Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGAAGACCCTGTTGAGCTTGACTCTAGTCTGGCACGGTGAAGAGACATGA610700.11649365283178484No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)