FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007948029

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007948029
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52423457
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGCCGCCCCGACCCTTCTCCCCCCGCCGCGCCCCCACGCGGCGCTCCC1732790.3305371486661019No Hit
CCTGCGGCGGCCTCCACCCGGGCCCGCGCCCTAGGCTTCAAGGCTCACCG1539400.2936471740121984No Hit
CCCACACCCCCGCCGCCGCCGCCGCCGCCGCCCTCCGACGCACACCACAC1453030.27717172486354724No Hit
CCGCGGCCCGCCCCCCGGCCGGGGCCGGAGAGGGGCTGACCGGGTTGGTT1282620.2446652840921956No Hit
CCCGCCCCGGGCCCCTCGCGGGGACACCGGGGGGGCGCCGGGGGCCTCCC1107140.21119171900471959No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG769190.1467263023115778Illumina Single End PCR Primer 1 (100% over 30bp)
CCGCCCCGGGCCCCTCGCGGGGACACCGGGGGGGCGCCGGGGGCCTCCCA754160.1438592651377417No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA745650.14223594601935544No Hit
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA698500.13324188063370182No Hit
CCGACGTCGCCGCCGACCCCGTGCGCTCGCTCCGCCGTCCCCCTCTTCGG645490.1231299950325672No Hit
CTCCCCCCGCCGCGCCCCCACGCGGCGCTCCCCCGGGGAGGGGGGAGGAC634310.12099736192521604No Hit
CCCCGCCCCGGGCCCCTCGCGGGGACACCGGGGGGGCGCCGGGGGCCTCC626180.11944652944196336No Hit
CCGGGCCCCTCGCGGGGACACCGGGGGGGCGCCGGGGGCCTCCCACTTAT613550.11703730259528669No Hit
CCCGTCGCCGGGGCGGGGGCGCGGGGAGGAGGGGTGGGAGAGCGGTCGCG597000.11388031888091622No Hit
CTTCTCCCCCCGCCGCGCCCCCACGCGGCGCTCCCCCGGGGAGGGGGGAG590830.11270336483151044No Hit
GCCGCCCCGACCCTTCTCCCCCCGCCGCGCCCCCACGCGGCGCTCCCCCG585800.11174387068750541No Hit
CTCGTCCAGCCGCGGCGCGCGCCCAGCCCCGCTTCGCGCCCCAGCCCGAC573030.1093079382384111No Hit
CTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACA557730.10638939740276954No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)