FastQCFastQC Report
Sat 18 Mar 2023
EGAF00007948030

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007948030
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76865447
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1016110.13219333779454895No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTAGAACC973230.12661475838421912TruSeq Adapter, Index 16 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTAGAACA819490.10661357371667922TruSeq Adapter, Index 16 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTAGACAA811670.10559621152011255TruSeq Adapter, Index 16 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTAGACAC782100.10174922940342752TruSeq Adapter, Index 16 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTAGAAAC769780.10014642860269843TruSeq Adapter, Index 16 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)