FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007948036

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007948036
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28863071
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG1590150.5509289015018534Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACTAA1191740.4128943867407595TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACTCA1128600.39101868266200784TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACACT1101090.38148747234831665TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACAAA1060890.3675596404831627TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACACA1058840.3668493903507357TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACAAT1034260.35833331803119634TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACTAC984260.3410101440695621TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACTAT960110.33264305104609276TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACCAA947390.32823603559025305TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACAAC945860.32770594646702705TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACTCT945780.3276782293886884TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACCTC934450.3237527981689821TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACCAT917170.3177659092478413TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACATC912910.31628997482631005TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACTTA885050.30663750229488745TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACCCA877610.3040598140093963TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACATT876260.3035920883124322TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACACC872060.30213694169965494TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACATA857200.2969884943982572TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACCTA831160.2879665853990381TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACGCA827900.28683711445673954TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACCCT799630.27704259189883157TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACGCT792660.2746277414485797TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACTTT752580.26074148520093376TruSeq Adapter, Index 20 (97% over 37bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA742560.25726992113902225No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACCAC732950.2539404071035961TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACCTT674650.23374158626433064TruSeq Adapter, Index 20 (97% over 37bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACA674280.23361339477701457TruSeq Adapter, Index 18 (97% over 34bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACTGC665300.23050215273350502TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACTCC637210.22076999360185895TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACCCC630940.21859766758707003TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACTTC608890.21095814786998932TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACGCC588980.20406005999846658TruSeq Adapter, Index 20 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACAA574780.19914027859336245TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACTGA547820.18979962319324925TruSeq Adapter, Index 20 (97% over 37bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCG546030.18917945356542276TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACGTC542020.18779013501369968TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACGTA534280.1851085076844387TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACGAA527520.18276641456482576TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACAAG527440.18273869748648713TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACTAG522930.18117614719514774TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACAGC521000.18050747268022865TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACATG500770.17349851649535145TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACAGT497210.1722651065092831TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACGTT476840.1652076454373133TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACCGC455140.15768938793796405TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACGAT451650.15648023039544198TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACTGT450390.15604368641160882TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACTTG444690.1540688445799825TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACAGA428900.14859818624289842TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACTCG412600.14295083153140564TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACCGT409470.14186640084140736TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACCAG408310.14146450320549744TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACGAC403990.13996778097521223TruSeq Adapter, Index 20 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACTA397470.13770883909061513TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACACG396390.13733465853304383TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACGGC353530.12248523381313096TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACGGT352940.12228082036038368TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACCTG346130.11992140406680912TruSeq Adapter, Index 20 (97% over 37bp)
AAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT341610.11835538914067736Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTA330420.11447846280806363TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACCGA321160.11127021099036898TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACCCG303530.10516205985149675TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACAGG292650.10139253719744513TruSeq Adapter, Index 20 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)