FastQCFastQC Report
Wed 15 May 2024
EGAF00007948042

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007948042
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55947001
Total Bases8.4 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG2907170.5196292827206234Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCAAAA2091950.37391637846682796TruSeq Adapter, Index 12 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCAAA1978330.35360787256496556TruSeq Adapter, Index 12 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCAAAT1646930.2943732408462788TruSeq Adapter, Index 12 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCATAA1645520.29412121661355894TruSeq Adapter, Index 12 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCAAAC1618890.2893613546863754TruSeq Adapter, Index 12 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCAATA1513330.27049349794459937TruSeq Adapter, Index 12 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCACAA1481940.26488283080624825TruSeq Adapter, Index 12 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCAACA1444950.258271216360641TruSeq Adapter, Index 12 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCAATT1280630.2289005625163TruSeq Adapter, Index 12 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCAACT1265980.22628201286428204TruSeq Adapter, Index 12 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCATAT1247490.22297709934443136TruSeq Adapter, Index 12 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCATTA1196220.2138130692653213TruSeq Adapter, Index 12 (97% over 37bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1137580.20333172103362607No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCAATC1124730.2010349044446547TruSeq Adapter, Index 12 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCATCA1121930.20053443079102667TruSeq Adapter, Index 12 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCAGAA1113220.1989776002470624TruSeq Adapter, Index 12 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCATAC1065220.19039805189915363TruSeq Adapter, Index 12 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCAAAG1064620.1902908075448048TruSeq Adapter, Index 12 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCATA999300.1786154721680256TruSeq Adapter, Index 12 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCACTA998580.17848677894280696TruSeq Adapter, Index 12 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCATCT984000.17588074113212968TruSeq Adapter, Index 12 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCAACC977760.17476539984690154TruSeq Adapter, Index 12 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCACAT968880.1731781834025384TruSeq Adapter, Index 12 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCAAT962630.17206105471140445TruSeq Adapter, Index 12 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCACCA882270.15769746085228054TruSeq Adapter, Index 12 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCATTT870020.155507888617658TruSeq Adapter, Index 12 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCACAC829550.1482742569168274TruSeq Adapter, Index 12 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCAGTA825560.1475610819604075TruSeq Adapter, Index 12 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCATGA806140.1440899396913161TruSeq Adapter, Index 12 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCAATG756870.13528339079336887TruSeq Adapter, Index 12 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCATTC739390.13215900527000546TruSeq Adapter, Index 12 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCATAG738260.13195702840264845TruSeq Adapter, Index 12 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCACCT719310.1285698942111303TruSeq Adapter, Index 12 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCAAGA706660.12630882574027516TruSeq Adapter, Index 12 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCAGCA704550.12593168309414832TruSeq Adapter, Index 12 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCAAGT683930.1222460521163592TruSeq Adapter, Index 12 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCAGAT672550.12021198419554248TruSeq Adapter, Index 12 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCAA666600.11914847768158296TruSeq Adapter, Index 12 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCAAGC662890.11848535009052585TruSeq Adapter, Index 12 (97% over 37bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATG662310.11838168054798862TruSeq Adapter, Index 12 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCACTT648700.11594902111017533TruSeq Adapter, Index 12 (97% over 37bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGC640390.11446368680244362TruSeq Adapter, Index 12 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCATGC614010.10974851002290542TruSeq Adapter, Index 12 (97% over 37bp)
GGAGGATTCAACCCGGCGGCGGGTCCGGCCGTGTCGGCGGCCCGGCGGAT607750.10862959392586567No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCAGCT601120.10744454381031077TruSeq Adapter, Index 12 (97% over 37bp)
GAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG582590.10413248066683681No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCATCC575930.10294206833356447TruSeq Adapter, Index 12 (97% over 37bp)
CGGAGCCCCGCGGACGCTACGCCGCGACGAGTAGGAGGGCCGCTGCGGTG575310.10283124916740398No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCATTG568740.10165692348728396TruSeq Adapter, Index 12 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCATGT565400.101059929914742TruSeq Adapter, Index 12 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)