FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007948046

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007948046
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53131389
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4489660.8450108466014317No Hit
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCAA1904540.35845853756994756TruSeq Adapter, Index 15 (97% over 37bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG1848370.347886632513974Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCAAC1504840.28322993776804894TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCACT1448390.27260533316755564TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCAAA1307040.24600147381804754TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCAAT1276450.24024404857926826TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCCAA1246610.23462778283473823TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCTAA1191690.22429114360251337TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCATT1183910.22282684911550119TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCACA1180040.2220984661251751TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCATA1177210.2215658243002079TruSeq Adapter, Index 15 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCTA1148850.21622811329099642TruSeq Adapter, Index 15 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCAT1142920.21511201222313234TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCTCT1137130.21402226092752816TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCCAT1132830.2132129464938325TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCATC1122100.21119342466277327TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCTTA1114520.20976677270756086TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCCTA1109280.2087805383744061TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCTAC1010970.19027735186821484TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCTAT1001240.18844604269615461TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCCAC989540.18624395458586637TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCACC977170.1839157639940488TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCTTT963120.1812713761351129TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCTCA959820.18065027436041622TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCCTT936080.17618210583578006TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCCCT913590.17194920313489262TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCTGC872560.16422683773616384TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCTGT869190.16359256107533723TruSeq Adapter, Index 15 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCCA865720.1629394631486107TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCCCA855080.1609368804568614TruSeq Adapter, Index 15 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCAC802660.15107077287213402TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCTTC800420.15064917651597629TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCCTC796680.14994526117132004TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCTGA796600.1499302041586001TruSeq Adapter, Index 15 (97% over 37bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGAC776930.14622806115608986TruSeq Adapter, Index 15 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCA729220.13724843519524776TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCATG710060.13364228064882702TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCGCT703250.13236055244104386TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCAGC700960.13192954545193614TruSeq Adapter, Index 15 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCTT678630.12772675677648856TruSeq Adapter, Index 15 (97% over 37bp)
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA633120.11916119866544425No Hit
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCCT610870.1149734670027166TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCCCC605960.11404934284703154TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCGAC602340.11336801302145517TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCAGT583960.10990866434905362TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGAC582010.10954164966400558TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCTTG581880.10951718201833573TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCCTG578590.10889796237022903TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCGCA571520.10756729887110611TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCTCC559650.10533321460878804TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCGAA557860.10499631394917984TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCTAG555180.10449190402306253TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCGTA550600.10362989004484711TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCAAG535570.10080105378009223TruSeq Adapter, Index 15 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)