FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007948047

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007948047
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53131389
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2863930.5390278804869942No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2566170.4829856791434532No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG2117840.39860429773443345Illumina Single End PCR Primer 1 (100% over 30bp)
CCTGCCGCCCCGACCCTTCTCCCCCCGCCGCGCCCCCACGCGGCGCTCCC1235220.23248404064873965No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA815210.1534328417425714No Hit
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAAGTCGAGGTGT760280.14309432038375658Illumina Single End PCR Primer 1 (97% over 34bp)
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA562750.10591667385168493No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)