FastQCFastQC Report
Tue 14 May 2024
EGAF00007948050

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007948050
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70983286
Total Bases10.7 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG1188970.1674999942944315Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC958520.13503460518860735No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT909730.12816115613469908No Hit
CTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC834700.11759106221146201No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)