FastQCFastQC Report
Wed 22 May 2024
EGAF00007948056

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007948056
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96731789
Total Bases14.6 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG3171490.32786429702028974Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1152670.1191614475361352No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)