FastQCFastQC Report
Mon 3 Apr 2023
EGAF00007948058

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007948058
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35916796
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG17583774.89569559601029Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3328700.9267808854665098No Hit
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAAC2257350.6284942565589648TruSeq Adapter, Index 6 (97% over 36bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAACT1623330.45196960218834664TruSeq Adapter, Index 6 (97% over 36bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAACTA1578050.43936268702809683TruSeq Adapter, Index 6 (97% over 36bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACG1563790.435392399700686TruSeq Adapter, Index 6 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAACTAA1475410.41078552775141747TruSeq Adapter, Index 6 (97% over 36bp)
ACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAA1200300.3341890518296788TruSeq Adapter, Index 6 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAACTTA1020950.2842541968387158TruSeq Adapter, Index 6 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAACTAC994540.27690109106614075TruSeq Adapter, Index 6 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAACTAT969260.26986260133002954TruSeq Adapter, Index 6 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAACTGA913490.254335047034819TruSeq Adapter, Index 6 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAACTGC887700.2471545624503923TruSeq Adapter, Index 6 (97% over 36bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAACTT866590.24127708941521397TruSeq Adapter, Index 6 (97% over 36bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAACTG776880.21629991717524022TruSeq Adapter, Index 6 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAACTCA687820.191503718761551TruSeq Adapter, Index 6 (97% over 36bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAACTC674140.1876949157714402TruSeq Adapter, Index 6 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAACTAG671890.18706846791122458TruSeq Adapter, Index 6 (97% over 36bp)
GACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGA656490.18278078033463788TruSeq Adapter, Index 6 (97% over 36bp)
AAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT644340.17939796188947366Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAACTGT640060.178206318848708TruSeq Adapter, Index 6 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAACTTT615500.17136829242786578TruSeq Adapter, Index 6 (97% over 36bp)
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC585360.1629766753136889Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACGACGAACTA542330.15099620801365465TruSeq Adapter, Index 9 (96% over 33bp)
CCTCCACCCGCCCTCCCTTCCCCCGCCGCCCCTCCTCCTCCTCCCCGGAG538800.1500133809262942No Hit
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAACTCT528540.14715677868371108TruSeq Adapter, Index 6 (97% over 36bp)
AAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAC508990.1417136428316156TruSeq Adapter, Index 6 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAACTTG446780.12439305554983245TruSeq Adapter, Index 6 (97% over 36bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACGACGAACTT441030.1227921332403926TruSeq Adapter, Index 9 (96% over 33bp)
CAAGCAGAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT434770.1210492160826372Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTCCACCCGCCCTCCCTTCCCCCGCCGCCCCTCCTCCTCCTCCCCGGAGG416410.11593740154327797No Hit
CCCGGCCCCGTCCGCCCCCCGTTCCCCCCTCCTCCTCGGCGCGCGGCGGC398650.11099263976664289No Hit
CTTCCCCCGCCGCCCCTCCTCCTCCTCCCCGGAGGGGGCGGGCTCCGGCG391640.1090409066554823No Hit
GAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA384410.10702792086465619TruSeq Adapter, Index 7 (97% over 35bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAACTTC382380.10646272568410613TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAACTGCC377390.10507340354078352TruSeq Adapter, Index 6 (97% over 36bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACACCAG374610.10429939240682827Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
AGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG368790.10267898060840393Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)