FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007948059

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007948059
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35916796
Sequences flagged as poor quality0
Sequence length151
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG18877255.255827941891031Illumina Single End PCR Primer 1 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2220050.6181091431429463No Hit
CCTGCCGCCCCGACCCTTCTCCCCCCGCCGCGCCCCCACGCGGCGCTCCC1795190.49981908185796975No Hit
CCCACACCCCCGCCGCCGCCGCCGCCGCCGCCCTCCGACGCACACCACAC1437960.40035865114471786No Hit
CCTGCGGCGGCCTCCACCCGGGCCCGCGCCCTAGGCTTCAAGGCTCACCG1435610.3997043611573816No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1396220.3887373472845406No Hit
CGTATGCCGTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCGCA818200.22780428410151063Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCCGTCTTCTGCTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT673700.18757241041210915Illumina Single End PCR Primer 1 (100% over 31bp)
CGTATGCCGTCTTCTGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG603950.16815252674542575Illumina Single End PCR Primer 1 (100% over 32bp)
CTTCTCCCCCCGCCGCGCCCCCACGCGGCGCTCCCCCGGGGAGGGGGGAG578730.16113074228558694No Hit
CTCCCCCCGCCGCGCCCCCACGCGGCGCTCCCCCGGGGAGGGGGGAGGAC570330.15879200360744872No Hit
GGCGGACCCGGCGGGGGGGACCGGCCCGCGGCCCCTCCGCCGCCTGCCGC558830.155590158988569No Hit
GGGGGACCGGCCCGCGGCCCCTCCGCCGCCTGCCGCCGCCGCCGCCGCGC520310.14486537162167806No Hit
GCGGACCCGGCGGGGGGGACCGGCCCGCGGCCCCTCCGCCGCCTGCCGCC493290.1373424288736668No Hit
GCCGCCCCGACCCTTCTCCCCCCGCCGCGCCCCCACGCGGCGCTCCCCCG479600.13353084167084392No Hit
CTCCCCGGGGGCGGCCGCGACGCCCGCCGCAGCTGGGGCGATCCACGGGA479110.1333944152479525No Hit
CCCGTCGCCGGGGCGGGGGCGCGGGGAGGAGGGGTGGGAGAGCGGTCGCG467680.1302120601180573No Hit
CGGCCCGCGGCCCCTCCGCCGCCTGCCGCCGCCGCCGCCGCGCGCCGAGG464990.12946310689851065No Hit
CCACCCCCGGCCCCGCCCGCCCACCCCCGCACCCGCCGGAGCCCGCCCCC463960.12917633298916753No Hit
CCCGCCCCGGGCCCCTCGCGGGGACACCGGGGGGGCGCCGGGGGCCTCCC441650.1229647544285409No Hit
CGGTCCCGCCGCCCCCGCCGCCGCCGCCACCGCCGCCGCCGCCGCCGCCC439380.12233273814290116No Hit
CGCCGCCCCCGCCGCCGCCGCCACCGCCGCCGCCGCCGCCGCCCCGACCC429940.11970444134270775No Hit
CCCGGCCCCGCCCGCCCACCCCCGCACCCGCCGGAGCCCGCCCCCTCCGG427570.11904458292994731No Hit
TCCCCCGCCGACCCCACCCCCGGCCCCGCCCGCCCACCCCCGCACCCGCC426280.11868541949009037No Hit
CCGGGCCCGACGGCGCGACCCGCCCGGGGCGCACTGGGGACAGTCCGCCC398070.11083115542934287No Hit
CGTATGCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCGCATTG398010.11081445015307045Illumina Single End PCR Primer 1 (96% over 33bp)
CCGACGTCGCCGCCGACCCCGTGCGCTCGCTCCGCCGTCCCCCTCTTCGG388270.10810262697151495No Hit
CCCCACGCGGCGCTCCCCCGGGGAGGGGGGAGGACGGGGAGCGGGGGAGA373020.10385670258560925No Hit
CCCCCCGCCGCGCCCCCACGCGGCGCTCCCCCGGGGAGGGGGGAGGACGG371450.10341958118981437No Hit
CGTATGCCGTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCGCAT371000.10329429161777125Illumina Single End PCR Primer 1 (96% over 33bp)
CCCGCCGCGCCCCCACGCGGCGCTCCCCCGGGGAGGGGGGAGGACGGGGA360910.10048502099129332No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)