FastQCFastQC Report
Mon 13 May 2024
EGAF00007948060

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007948060
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59282275
Total Bases8.9 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG7734741.304730629855214Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2910310.4909241421655967No Hit
GGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA1352460.22813901794423377No Hit
GGGGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA1068110.18017358476880316No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA871240.14696467029984933No Hit
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCA859210.14493539595098198TruSeq Adapter, Index 6 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCACGTTAA809140.13648936381068372TruSeq Adapter, Index 6 (97% over 37bp)
GGGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG594680.10031328925888894No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)