FastQCFastQC Report
Mon 13 May 2024
EGAF00007948061

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007948061
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59282275
Total Bases8.9 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG8828851.4892900112217353Illumina Single End PCR Primer 1 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2060860.3476351067836044No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1892730.3192741843999745No Hit
GGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA1197600.20201653867028552No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA916740.15463981434585633No Hit
GGGGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA868430.1464906668983267No Hit
GCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC780130.13159582691453728No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA715270.12065495124807542No Hit
CCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC659050.11117150952793226No Hit
CGTATGCCGTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTTCA616510.10399567155612703Illumina Single End Sequencing Primer (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)