FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007948068

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007948068
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30961758
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG6784202.1911546495518763Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3166401.022680947251122No Hit
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACA2711560.8757771441789578TruSeq Adapter, Index 15 (97% over 36bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACAC1669670.5392684743547185TruSeq Adapter, Index 15 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACTA1484930.4796013197958591TruSeq Adapter, Index 15 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACTT1478260.47744704935682275TruSeq Adapter, Index 15 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACAT1405080.4538114405519222TruSeq Adapter, Index 15 (97% over 36bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACT1320000.42633238073884566TruSeq Adapter, Index 15 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACTC1307550.42231129123869515TruSeq Adapter, Index 15 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACAC1199890.38753936388237387TruSeq Adapter, Index 15 (97% over 36bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACA1156790.37361896569309794TruSeq Adapter, Index 15 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACAA1142760.3690875692523661TruSeq Adapter, Index 15 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACTG1104650.3567788366539135TruSeq Adapter, Index 15 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACGC1101540.3557743717265667TruSeq Adapter, Index 15 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACGT1099840.3552253072968273TruSeq Adapter, Index 15 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACCT1080610.3490144196592454TruSeq Adapter, Index 15 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACCA958710.30964327025616567TruSeq Adapter, Index 15 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACAG926290.2991722886019586TruSeq Adapter, Index 15 (97% over 36bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACG897670.2899286274377572TruSeq Adapter, Index 15 (97% over 36bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACA883040.28520344355123506TruSeq Adapter, Index 13 (96% over 33bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGA803370.2594717005410352TruSeq Adapter, Index 15 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACCC781540.2524210673050284TruSeq Adapter, Index 15 (97% over 36bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACC775820.25057362698849334TruSeq Adapter, Index 15 (97% over 36bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACT705270.22778745315430732TruSeq Adapter, Index 13 (96% over 33bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACGA696310.22489356063050428TruSeq Adapter, Index 15 (97% over 36bp)
GACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAA646950.20895131342348197TruSeq Adapter, Index 15 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACGG613720.19821871871745783TruSeq Adapter, Index 15 (97% over 36bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACC566340.1829159700815438TruSeq Adapter, Index 13 (96% over 33bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACCG545030.176033285965222TruSeq Adapter, Index 15 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACTCT526320.16999034744732516TruSeq Adapter, Index 15 (97% over 36bp)
ACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAAC426300.1376859802340681TruSeq Adapter, Index 15 (97% over 36bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACG413350.13350340119575899TruSeq Adapter, Index 13 (96% over 33bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACCTC401650.12972454600284647TruSeq Adapter, Index 15 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACTGC399120.12890740893976368TruSeq Adapter, Index 15 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACTTT397340.1283325061839189TruSeq Adapter, Index 15 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACGCT392210.1266756235224111TruSeq Adapter, Index 15 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACACT327870.10589514975215554TruSeq Adapter, Index 15 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACTCA310510.10028823298728709TruSeq Adapter, Index 15 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)