FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007948070

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007948070
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37072957
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG16324924.403457754934412Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCC4620921.2464395543090885TruSeq Adapter, Index 12 (97% over 34bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCA4025571.0858507995464186TruSeq Adapter, Index 12 (97% over 34bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAG3961711.068625305502337TruSeq Adapter, Index 22 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCTA3513170.9476368448300468TruSeq Adapter, Index 22 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCAA3370350.9091128069444258TruSeq Adapter, Index 22 (97% over 38bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3325580.8970366189025601No Hit
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCAT3279620.8846394421680472TruSeq Adapter, Index 22 (97% over 38bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCT3121430.8419695251177294TruSeq Adapter, Index 12 (97% over 34bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCTC2320780.6260034774134688TruSeq Adapter, Index 22 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCAC2268140.611804448185776TruSeq Adapter, Index 22 (97% over 38bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGC2146890.5790986675273839TruSeq Adapter, Index 22 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCTT2137570.5765847056656419TruSeq Adapter, Index 22 (97% over 38bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCG2077130.5602817169399247TruSeq Adapter, Index 12 (97% over 34bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCCA1772140.47801420318319904TruSeq Adapter, Index 22 (97% over 38bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCA1687520.45518894001360616TruSeq Adapter, Index 22 (97% over 38bp)
ACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACA1613870.4353227070611066TruSeq Adapter, Index 22 (97% over 38bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCT1541670.4158475947845218TruSeq Adapter, Index 22 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCGT1411270.3806737077919088TruSeq Adapter, Index 22 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCCT1375330.37097930979716565TruSeq Adapter, Index 22 (97% over 38bp)
CAAGCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGC1220650.3292561745209588TruSeq Adapter, Index 22 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCCC1192460.32165224910438084TruSeq Adapter, Index 22 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCGC1175680.31712603879965656TruSeq Adapter, Index 22 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCAG1158300.3124379854566227TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCTCT1142200.30809519726198265TruSeq Adapter, Index 22 (97% over 38bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTA1080510.29145503553978713TruSeq Adapter, Index 22 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCC1071400.2889977187414535TruSeq Adapter, Index 22 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCGA1017410.2744345426775641TruSeq Adapter, Index 22 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCTG1016380.27415671212846604TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCGCT866750.23379575575803138TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCTTT845040.22793973515519683TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCTCA830340.2239745807166124TruSeq Adapter, Index 22 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCCG803940.21685348703099136TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCACT792850.21386208820623615TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCTTC781850.2108949658372274TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCTAC777940.20984028870424334TruSeq Adapter, Index 22 (97% over 38bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCG763690.2059965165443911TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCATC751460.20269761594684774TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCCCT749300.20211498100893327TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCTCC722000.19475112276584788TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCTGC683550.18437968139417635TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCTAT679750.18335467548488243TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCCTC668650.1803605792761554TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCCTT653280.1762147001114586TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCATT642220.17323139343861887TruSeq Adapter, Index 22 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCGG634180.17106269672527066TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCTTA619450.16708945013477075TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCCCC596720.16095829636680992TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCTAA592550.1598334872505584TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCAAA590780.15935605028754518TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCATA584670.15770794868075938TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCGTC580250.15651570496521225TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCAAT570930.15400174310347028TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCCAC564790.15234554934476902TruSeq Adapter, Index 22 (97% over 38bp)
GACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTAC563730.15205962664375544TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCTCG561290.15140146495462986TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCACA541840.146155053129428TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCGTT528520.1425621376789556TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCACC514190.1386967864473287TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCGCA514000.138645536151864TruSeq Adapter, Index 22 (97% over 38bp)
TAAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAG511390.1379415189352174No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCTTG508660.13720513311090884TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCCCA505660.136395917919361TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCGGC503950.13593466526017872TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCGCC502120.13544104399333456TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCCTA484560.13070443773880783TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCCGC474110.1278856714882495TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCTGT468980.1265019135107027TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCCAT460150.1241201234635802TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCAGC440210.11874153982375886TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCCAA440160.11872805290389973TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCCGT434510.11720403095981796TruSeq Adapter, Index 22 (97% over 38bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACACCAG432100.11655396142260786Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCAAC423130.1141344079998798TruSeq Adapter, Index 22 (97% over 38bp)
GGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCA399810.10784410857758123No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCCTG372590.10050182940627046TruSeq Adapter, Index 22 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)