FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007948072

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007948072
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61835944
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATGGCACATCTCGTAT9645291.5598193180328905TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATGGCACATCGCGTAT3570440.5774052709537353TruSeq Adapter, Index 9 (97% over 36bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1696810.2744051259248181No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATGGCACATCTCGTA653900.10574755679318165TruSeq Adapter, Index 9 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)