FastQCFastQC Report
Fri 10 May 2024
EGAF00007948074

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007948074
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11782817
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATGGCACATCTCGTAT1836151.5583285389223986TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATGGCACATCGCGTAT677230.5747606875333802TruSeq Adapter, Index 9 (97% over 36bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA269020.22831552081306195No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATGGCACATCTCGTA121090.10276829386385276TruSeq Adapter, Index 9 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)