FastQCFastQC Report
Wed 8 May 2024
EGAF00007948076

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007948076
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7709975
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATGGCACATCTCGTAT1148931.4901864143528352TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATGGCACATCGCGTAT580140.7524538017308746TruSeq Adapter, Index 9 (97% over 36bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA221350.2870956131504966No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATGGCACATCTCGTA77260.10020784762596506TruSeq Adapter, Index 9 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)