FastQCFastQC Report
Wed 31 May 2023
EGAF00007948436

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007948436
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences524291
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA77911.4860068168250073No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA70871.3517302414117351No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT34480.6576500454900046No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC25100.4787417674535706No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC23300.4444096885126771No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA22980.4383062078120738No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT21590.4117942135188283No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT17400.33187676309530395No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA14090.2687438845984387No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT13440.2563461894253382No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT13170.2511963775842042No Hit
GATTTCCGGGGTTTTGACAGAACGCAGCAGGTCAGCGATGGCCG13050.24890757232147795No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT12300.23460253942943898No Hit
TTCCTCACCGGCAGCTTTTGCCTGTTCTTCAATGTTAGCAACAA10710.20427586969831638No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT10460.19950752540097005No Hit
GTCGGAAATCTGTTTCTCTTCCTTAACTTTATCCAGTTCCGCGG10290.19626505127877455No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG9720.18539322628082494No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG9480.18081561575537247No Hit
CTTGCGAGACGCTGCGCTAGTAGGGGCAACCGCCGGGTCCGGTT9040.17242332979204297No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG8470.16155150479409336No Hit
TGGAATGGTTTTGCAGATGGATTTGCACAGCTGGTTGTTACCGC7290.13904491971061872No Hit
TACGGTATCAGGGGATTCCGGCTGCACCTCGGTTTCGCCTTTGT7120.13580244558842322No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG6960.13275070523812157No Hit
CGGAGACAGCTGAGAGTATGGGATGAAATGGTAGTGATCGCCGT6960.13275070523812157No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG6840.13046189997539534No Hit
TTCTTCAGTGTGGTCCTCTTCAGATTTTTTCTTGTCCTCGCTCT6630.12645649076562443No Hit
TGGGATAACATCGGTCGGAATGTAGGACAGAGATTCCTCCGCGT6630.12645649076562443No Hit
GCCTTGACGAGATGCGTCGGAAATCTGTTTTTCTTCTTTGACCT6630.12645649076562443No Hit
GGTACCGCTGTTCAGGGTGTACTGCTGGAACAGCTGCTTCCACG6270.11959007497744573No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG6240.11901787366176417No Hit
GGTCGGTTTTTTCTTCGGTTTGTTGGACGGAATGGTCTTGCAGA6240.11901787366176417No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG6190.1180642048022949No Hit
GTCAACCTGGTTGTCGTTCGCGATTTTCTCGGTGATAGCTTCCA6120.11672906839903793No Hit
TCAACCAATGTGTGGGGTAGTTGCGACGGTGTCTACCAGGATGC5820.11100705524222235No Hit
GATTTCGTTAACGATATCGCTGTCCTTCAGCTGATAGTTCGGGT5610.10700164603245144No Hit
GGGCACTATCGAGCAACGCCTTCATTGCATCCGAGTAAGTATGA5590.10662017848866373No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA5420.10337770436646823No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG5380.10261476927889282No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)