FastQCFastQC Report
Wed 31 May 2023
EGAF00007948437

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007948437
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences524291
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT179543.424434140582234No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG49080.9361213524550297No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC36640.6988485402190767No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT34070.6498299608423567No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA24270.46291086438638085No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA22450.4281973179016996No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT15530.2962095477511535No Hit
ACCCTGTCCCTGTCTCAGCTGTACTCCCACA13990.26683654687950015No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG13840.2639755403010923No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA13590.25920719600374603No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC13070.24928903986526565No Hit
ACTACCGACCCGGATGCAGCGGCTTCCGCGA10930.20847201267998114No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG10790.2058017398734672No Hit
AAACAAGATTTCGGTGCGCTGAAACAGGAGC10730.20465733724210408No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC9450.1802434144396909No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT9360.17852681049264624No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG9320.17776387540507083No Hit
CCGGCCGTGGCACCAACCAGCGCTGCCTCCC9270.17681020654560156No Hit
GATGTCCAGTCTGGTGCGATCGTTGAACCGG8030.15315921883076383No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG7880.15029821225235604No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG7540.14381326400796504No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC7390.14095225742955725No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG7000.133513640325697No Hit
ATGACCCAGGGTCGCCGCGGTAACGTTTACT6930.13217850392244002No Hit
TATCACCAGAACCAGGGTGAAAACATTTCCA6840.13046189997539534No Hit
AACCTGACCGCGGAACTGGATAAAGTTAAAG6820.13008043243160763No Hit
ACCACCAACGAACAGCCGAAACCAGAATCTT6740.1285545622564568No Hit
CCGAGCACTAAACCGCGTCCGAAAAACCCTC6680.1274101596250937No Hit
GTGCGCCAGGAAAACCTGCAGATTCTGATGA6680.1274101596250937No Hit
CCGAAAGACCAGTTTCTGGAAGCAATCACCG6670.12721942585319984No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG6460.12321401664342893No Hit
ACCACCACTTCTACTCAGACGAATAAACCGA6340.1209252113807027No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG6150.11730126971471949No Hit
CCGGACCCAGCGGTGGCACCGACTTCTGCGG6010.11463099690820555No Hit
GGCACTTCCGCTTTCGGTGATTCCGCGGACC6010.11463099690820555No Hit
CAGATCGTGATCAAAATTACCGATCAGGGTT5930.11310512673305473No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC5760.10986265261085923No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG5410.10318697059457438No Hit
GTTGGCAAAAGCATTGAAGATGCTCGTGAAG5310.10127963287563585No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)